| 货号: S217724 |
| 产品全名: PTRH2 兔多抗 |
| 基因符号 BIT1; PTH2; CFAP37; CGI-147 |
| UNIPROT ID: Q9Y3E5 (Gene Accession – BC006807 ) |
| 背景: PTRH2 (peptidyl-tRNA hydrolase 2), also known as BIT1 (Bcl-2 inhibitor of transcription 1), is a 179 amino acid mitochondrial protein. During apoptosis, PTRH2 is released from the mitochondia to the cytoplasm. Once in the cytoplasm, PTRH2 regulates the function of two transcriptional regulators, TLE5 and TLE1, thereby promoting caspase-independent cell death. It is also believed that the natural substrate for PTRH2 may be petidyl-tRNAs, which leave the ribosomes during protein synthesis. |
| 抗原: Fusion protein of human PTRH2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: Oct-50;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Cancer, Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 217724(PTRH2 Antibody) at a dilution of 1/20(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 217724(Anti-PTRH2 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 217724(Anti-PTRH2 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D222953(Anti-PTRH2 Antibody) at dilution 1/20. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: K562 cells, hepg2 cells, 231 cells, Jurkat cells, Raji cells;
Primary antibody: 217724(PTRH2 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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