| 货号: S217659 |
| 产品全名: NUP50 兔多抗 |
| 基因符号 NPAP60; NPAP60L |
| UNIPROT ID: Q9UKX7 (Gene Accession – BC016055 ) |
| 背景: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the Cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. |
| 抗原: Fusion protein of human NUP50 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 200-1000;ELISA: 1000-2000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Epigenetics and Nuclear Signaling, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 217659(NUP50 Antibody) at a dilution of 1/30(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 217659(Anti-NUP50 Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 217659(Anti-NUP50 Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D222796(Anti-NUP50 Antibody) at dilution 1/30. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Hela cells, 293T cells, A549 cells, HT29 cells, K562 cells;
Primary antibody: 217659(NUP50 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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