| 货号: S216781 |
| 产品全名: SLC12A1 兔多抗 |
| 基因符号 BSC1; NKCC2 |
| UNIPROT ID: Q13621 (Gene Accession – BC040138 ) |
| 背景: This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle’s loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven. |
| 抗原: Fusion protein of human SLC12A1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 200-1000;ELISA: 1000-2000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 216781(SLC12A1 Antibody) at a dilution of 1/15(Cytoplasm ). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 216781(Anti-SLC12A1 Antibody) at dilution 1/15. | 
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 216781(Anti-SLC12A1 Antibody) at a dilution of 1/15. | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D221227(Anti-SLC12A1 Antibody) at dilution 1/15. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human fetal kidney tissue;
Primary antibody: 216781(SLC12A1 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute | |
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