| 货号: S216779 |
| 产品全名: SKP1 兔多抗 |
| 基因符号 OCP2, p19A, EMC19, SKP1A, OCP-II, TCEB1L |
| UNIPROT ID: P63208 (Gene Accession – BC009839 ) |
| 背景: This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. |
| 抗原: Fusion protein of human SKP1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 216779(SKP1 Antibody) at a dilution of 1/30(Cytoplasm, Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 216779(Anti-SKP1 Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 216779(Anti-SKP1 Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with fusion protein and then with D221225(Anti-SKP1 Antibody) at dilution 1/30. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse brain tissue, NIH/3T3 cells;
Primary antibody: 216779(SKP1 Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
|
