武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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PRKCA Rabbit pAb

Anti-PRKCA Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S216733

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S216733
产品全名:   PRKCA 兔多抗
基因符号   AAG6; PKCA; PRKACA; PKC-alpha
UNIPROT ID:   P17252 (Gene Accession – BC109273 )
背景:   Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
抗原:   Fusion protein of human PRKCA
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 200-1000;ELISA: 2000-5000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Cardiovascular
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216733(PRKCA Antibody) at a dilution of 1/10(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216733(Anti-PRKCA Antibody) at dilution 1/10.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 216733(Anti-PRKCA Antibody) at a dilution of 1/10.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D221138(Anti-PRKCA Antibody) at dilution 1/10.

Gel: 10%SDS-PAGE, Lysate: 50 μg;
Lane 1-2: Jurkat cells, HT29 cells;
Primary antibody: 216733(PRKCA Antibody) at dilution 1/150;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
           引用文献