| 货号: S216628 |
| 产品全名: MAD2L1BP 兔多抗 |
| 基因符号 CMT2; RP1-261G23.6 |
| UNIPROT ID: Q15013 (Gene Accession – BC002904 ) |
| 背景: The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| 抗原: Fusion protein of human MAD2L1BP |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 216628(MAD2L1BP Antibody) at a dilution of 1/65(Cytoplasm or Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 216628(Anti-MAD2L1BP Antibody) at dilution 1/65. | 
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 216628(Anti-MAD2L1BP Antibody) at a dilution of 1/65. | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with fusion protein and then with D220942(Anti-MAD2L1BP Antibody) at dilution 1/65. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: A549 cells, 231 cells, PC3 cells, hela cells;
Primary antibody: 216628(MAD2L1BP Antibody) at dilution 1/675;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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