| 货号: S216604 |
| 产品全名: KIF3A 兔多抗 |
| 基因符号 FLA10; KLP-20 |
| UNIPROT ID: Q9Y496 (Gene Accession – BC045542 ) |
| 背景: Kinesin-like protein KIF3A is a protein that in humans is encoded by the KIF3A gene. Members of the heterotrimeric kinesin II family of microtubule associated motors generally contain two different motor subunits from the KIF3 family, which includes KIF3A, B and C. KIF3 isoforms mediate anterograde transport of membrane bound organelles in neurons and melanosomes, transport between the endoplasmic reticulum and the Golgi, and transport of protein complexes within cilia and flagella required for their morphogenesis. KIF3A may influence neurogenesis at the level of embryonic cellular events, where the asymmetry of the genetic control circuit controlling left-right (L-R) axis determination is defined. Loss of KIF3A function in mice photoreceptors causes apoptotic cell death, suggesting that kinesin II mediated transport is required for proper cell fate. |
| 抗原: Fusion protein of human KIF3A |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Neuroscience |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 216604(KIF3A Antibody) at a dilution of 1/40(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 216604(Anti-KIF3A Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 216604(Anti-KIF3A Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D220903(Anti-KIF3A Antibody) at dilution 1/40. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse brain tissue, human brain malignant glioma tissue;
Primary antibody: 216604(KIF3A Antibody) at dilution 1/750;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds | |
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