| 货号: S216471 |
| 产品全名: DAP3 兔多抗 |
| 基因符号 DAP-3; S29mt; MRPS29; MRP-S29; bMRP-10 |
| UNIPROT ID: P51398 (Gene Accession – BC107488 ) |
| 背景: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. |
| 抗原: Fusion protein of human DAP3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling , Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216471(DAP3 Antibody) at a dilution of 1/140(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216471(Anti-DAP3 Antibody) at dilution 1/140. | 
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 216471(Anti-DAP3 Antibody) at a dilution of 1/140. | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D220543(Anti-DAP3 Antibody) at dilution 1/140. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Jurkat, Hela, A549 and HepG2 cell lysates;
Primary antibody: 216471(DAP3 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute | |
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