| 货号: S216441 |
| 产品全名: COX7A2L 兔多抗 |
| 基因符号 EB1; SCAF1; SCAFI; SIG81; COX7AR; COX7RP |
| UNIPROT ID: O14548 (Gene Accession – BC007095 ) |
| 背景: Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein similar to polypeptides 1 and 2 of subunit VIIa in the C-terminal region, and also highly similar to the mouse Sig81 protein sequence. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. It is possible that this gene represents a regulatory subunit of COX and mediates the higher level of energy production in target cells by estrogen. Several transcript variants, some protein-coding and others non-protein coding, have been found for this gene. |
| 抗原: Fusion protein of human COX7A2L |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 216441(COX7A2L Antibody) at a dilution of 1/65(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 216441(Anti-COX7A2L Antibody) at dilution 1/65. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 216441(Anti-COX7A2L Antibody) at a dilution of 1/65. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D220471(Anti-COX7A2L Antibody) at dilution 1/65. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: A549, HepG2, Hela, RAW264.7 cell lysates;
Primary antibody: 216441(COX7A2L Antibody) at dilution 1/550;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 15 seconds | |
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