| 货号: S215843 |
| 产品全名: GPI 兔多抗 |
| 基因符号 AMF; NLK; PGI; PHI; GNPI; SA36; SA-36 |
| UNIPROT ID: P06744 (Gene Accession – NP_000166 ) |
| 背景: This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. |
| 抗原: Synthetic peptide of human GPI |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism, Cancer, Neuroscience, Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human esophagus cancer using 215843(GPI Antibody) at a dilution of 1/45(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer is first treated with the synthetic peptide and then with 215843(Anti-GPI Antibody) at dilution 1/45. | 
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer using 215843(Anti-GPI Antibody) at a dilution of 1/45. | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer is first treated with synthetic peptide and then with D163998(Anti-GPI Antibody) at dilution 1/45. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: PC3, Raji, Hela, 231 and Jurkat cell lysates;
Primary antibody: 215843(GPI Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes | |
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