武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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PLA2G4B Rabbit pAb

Anti-PLA2G4B Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S214254

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S214254
产品全名:   PLA2G4B 兔多抗
基因符号   HsT16992; cPLA2-beta
UNIPROT ID:   P0C869 (Gene Accession – NP_001108105 )
背景:   This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains.
抗原:   Synthetic peptide of human PLA2G4B
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism, Signal Transduction, Immunology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 214254(PLA2G4B Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 214254(Anti-PLA2G4B Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: BGC-823 cell lysate;
Primary antibody: 214254(PLA2G4B Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 minutes
           引用文献