| 货号: S213719 |
| 产品全名: PRMT5 兔多抗 |
| 基因符号 JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5 |
| UNIPROT ID: O14744 (Gene Accession – NP_001034708 ) |
| 背景: Protein arginine methyltransferase 5 has been shown to interact with WD repeat-containing protein 77, CLNS1A, Janus kinase 2, SNRPD3 and SUPT5H.Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. |
| 抗原: Synthetic peptide of human PRMT5 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 15-50;WB: 200-1000;ELISA: 1000-2000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 213719(PRMT5 Antibody) at a dilution of 1/20(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 213719(Anti-PRMT5 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 213719(Anti-PRMT5 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D160716(Anti-PRMT5 Antibody) at dilution 1/20. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: K562 cells, hela cells, 293T cells;
Primary antibody: 213719(PRMT5 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes | |
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