 
Figure 1. A. Flow cytometry analysis with anti-BCMA ( DM6) on K562-BCMA (Red histogram) (K562 cells stably transduced by human BCMA full length gene) and K562 (Negative control cell line) (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA ( DM6). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. |  
Figure 2. A. Flow cytometry analysis with anti-BCMA ( DM6) on NCI-H929 cells (Red histogram) or rabbit control antibody on NCI-H929 cells (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA ( DM6) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. |
Figure 3. Phylogenetic analysis of different Anti-BCMA DimAb clones. A) heavy chain and B) Light chain. | 
Figure 4. Affinity ranking of different DimAb clones by titration of rabbit DimAb antibody concentration onto K562-BCMA or NCI-H929 cells. Different concentrations of various anti-BCMA DimAb clones were incubated with K562-BCMA ( A) or NCI-H929 cells ( B) at 4. Bound rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. |
Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with FLAG tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-FLAG monoclonal antibody. | 
Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with FLAG tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-FLAG monoclonal antibody. |
