| 货号: 28395 |
| 产品全名: CD33(DM77) 兔单克隆抗体 |
| 基因符号 CD33;SIGLEC3;gp67 |
| 描述: CD33 antibody(DM77) 兔单克隆抗体 |
| 背景: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins; two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6:SHP-1 and PTPN11:SHP-2. In turn; these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase:PI3K. |
| 经过测试的应用: ELISA; Flow Cyt |
| 推荐稀释比: ELISA 1:5000-10000; Flow Cyt 1:100 |
| 种属反应性: Rabbit |
| 亚型: Rabbit IgG |
| 纯化: Purified from cell culture supernatant by affinity chromatography |
| 种属反应性: 人 CD33 |
| 成分: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD33 protein, hFc-His tagged protein 11166 can bind Rabbit anti-CD33 monoclonal antibody (clone: DM77) in a linear range of 1-100 ng/ml. | 
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CD33 (B) were surface stained with Rabbit anti-CD33 monoclonal antibody 1µg/ml (clone: DM77) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody. | 
Figure 3. Flow cytometry data of serially titrated Rabbit anti-CD33 monoclonal antibody (clone: DM77) on Expi 293 cell line transfected with human CD33. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. | |
|
