产品名称: KIT(V559D) 小鼠单抗 |
货号: 26277 |
基因符号: C-Kit; CD117; KIT; PBT; SCFR |
描述: KIT(V559D) 小鼠单抗 |
背景: Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 or Mast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism. |
免疫原: A synthetic peptide from the internal region of KIT which includes the mutation of V559D, human origin. |
经过测试的应用: ELISA, WB, IHC |
推荐稀释比: ELISA: 1:1000-1:5000WB: 1:500-1:1000IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
种属反应性: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG |
纯化: Purified from ascites |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Recognizes V559D mutant, but not wild type KIT of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Western blot analysis of recombinant KIT(V559D) and wild type proteins. Purified His-tagged KIT(V559D) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-KIT(V559D) monoclonal antibody (货号:26277).Immunofluorescence: Immunofluorescence of cells expressing KIT proteins with Anti-KIT(V559D) antibody.HEK293T cells were transfected with pCDNA3-GFP-KIT (WT) plasmid (left column) or pCDNA3-GFP-KIT(V559D) plasmid (right column), then fixed and stained with Anti-KIT(V559D) monoclonal antibody (货号:26277). |