| 产品名称: Arf6(Δ12Q67L) 突变蛋白 |
| 货号: 10125 |
| 产品全名: Arf6 Protein Δ12Q67L 突变蛋白 |
| 基因符号 ADP-ribosylation factor 6 |
| Source: Human, recombinant, His6-tag |
| Expression 种属反应性: E. coli |
| 分子量: 20 kDa |
| 纯化: >95% by SDS-PAGE |
| Introduction: Arf6 is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and the actin cytoskeketon at the plasma membrane and functions as a regulatory molecule of phagocytosis. |
| Amino Acid Sequence (1-175, Δ12, Q67L) |
MGKVLSKIFGN-EMRILMLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGL
DKIRPLWRHYYTGTQGLIFVVDCADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHE
IQEKLGLTRIRDRNWYVQPSCATSGDGLYEGLTWLTSNYKS |
Properties
|
| Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl. |
| Physical Appearance (form): White or clear |
| Concentration: 1mg/mL |
| Storage: -80°C |
Preparation Instructions:
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arf6 Δ12Q67L mutant was determined by SDS-PAGE and Coomassie Brilliant Blue Staining. |
References:
1. Cavenagh, M. M. et al., J. Biol. Chem. 271: 21767-21774, 1996.
2. D’Souza-Schorey, C. et al., Science 267: 1175-1178, 1995.
3. Falace, A. et al., Am. J. Hum. Genet. 87: 365-370, 2010.
4. Hernandez-Deviez, D. J. et al., Nature Neurosci. 5: 623-624, 2002.
5. O’Neal, C. J. et al., Science 309: 1093-1096, 2005.
