武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。
¥4,800.00
产品名称: Ras(G12D) 小鼠单抗 |
货号: 26036 |
基因符号: RAS(G12D) |
描述: Ras(G12D) 小鼠单抗 |
背景: Ras (G12D) is one type of Ras missense mutations at the codon 12. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G12D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. |
免疫原: A synthetic peptide from the internal region of Ras which includes the mutation of G12D, human origin |
经过测试的应用: ELISA, WB, IF, IHC |
推荐稀释比: ELISA: 1:100-1:3000WB: 1:100-1:1000IF: 1:50-1:100IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
种属反应性: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型:IgG1, IgG2a, IgG2b |
纯化: Purified from ascites |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Ras(G12D) antibody recognizes Ras(G12D) proteins of vertebrates |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Western blot analysis of recombinant Ras WT and G12D proteins.Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G12D (lane 2, Cat # 10154) proteins were loaded and blotted with anti-Ras G12D 小鼠单抗 (Cat # 26036) (Top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat # 21021).Immunofluorescence: Immunofluorescence of cells expressing RAS proteins with anti-RAS (G12D) antibody.HEK293T cells were transfected with pCDNA3-GFP-Ras(WT) plasmid (left column) or pCDNA3-GFP Ras(G12D) plasmid (right column), then fixed and stained with anti-Ras(G12D) monoclonal antibody (货号: 26036). |