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Mouse B7-H3 Protein, hFc Tag

Recombinant mouse B7-H3 protein with C-terminal human Fc tag
5/5

¥389.00

货号:12132

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   12132
产品全名:   Mouse B7-H3 蛋白
规格:   10/50/100 µg
基因符号   6030411F23Rik;AU016588;B7h3;B7RP-2
目标蛋白:    B7-H3
UNIPROT ID:    Q8VE98
描述:   Recombinant mouse B7-H3 protein with C-terminal human Fc tag
背景:     Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Plays a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells.[UniProtKB/Swiss-Prot Function]
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 28.7 kDa after removal of the signal peptide.The apparent molecular mass of mB7-H3-hFc is approximately 55-70 kDa due to glycosylation.
分子特征:   Mouse B7-H3(Val29-Phe244) hFc(Glu99-Ala330)
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Mouse B7-H3 Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Mouse B7-H3 Protein, hFc Tag  (12132) can bind anti-B7-H3 monoclonal antibody 28351 in a linear range of 3.2-400 ng/mL.
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