| 货号: 11593 |
| 产品全名: 人 IL4RA 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 CD124;IL-4RA;IL4RA |
| 目标蛋白: IL4RA |
| UNIPROT ID: P24394 |
| 描述: Recombinant human IL4RA Protein with C-terminal Human Fc tag |
| 背景: This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 49.9 kDa after removal of the signal peptide.The apparent molecular mass of IL4RA-hFc is approximately 55-70 kDa due to glycosylation. |
| 分子特征: IL4RA(Met26-His232) hFc(Glu99-Ala330) |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human IL4RA Protein, hFc Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human IL4RA Protein, hFc Tag(11593) can bind Anti-IL4RA antibody(DMC284), IgG1 Chimeric mAb in a linear range of 16.0-400 ng/mL. |
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