| 货号: 11311 |
| 产品全名: 人 CB1 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 CANN6;CB-R;CB1;CB1A;CB1K5;CB1R;CNR |
| 目标蛋白: CB1 |
| UNIPROT ID: P21554 |
| 描述: Recombinant Human CB1 protein with C-terminal human Fc |
| 背景: This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 44.2 kDa after removal of the signal peptide.The apparent molecular mass of CB1-hFc is approximately 70-100 kDa due to glycosylation. |
| 分子特征: CB1(Met1-Gln116) (Asp176-Asn187) (Asn256-Glu273) (Asp366-Thr377) hFc(Glu99-Ala330) |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CB1 Protein, hFc Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CB1 Protein, hFc Tag (11311) can bind Anti-CB1 antibody(DM144), Rabbit mAb in a linear range of 3.20-16 ng/mL. |
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