| 货号: 11282 |
| 产品全名: 人 CD162 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 PSGL-1;SGL1;D162;ELPLG;electin P ligand |
| 目标蛋白: CD162 |
| UNIPROT ID: Q14242 |
| 描述: Recombinant Human CD162 protein with C-terminal 6xHis tag |
| 背景: This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 31.4 kDa after removal of the signal peptide.The apparent molecular mass of CD162-His is approximately 70-100 kDa due to glycosylation. |
| 分子特征: CD162(Leu18-Cys320) 6×His tag |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CD162 Protein, His Tag on SDS-PAGE under reducing condition. |
