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Human GITR Protein, mFc-His tag

Recombinant human GITR protein with C-terminal mouse Fc and 6XHis tag
5/5

¥445.00

货号:11147

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11147
产品全名:   人 GITR 蛋白
规格:   10/50/100 µg
基因符号   AITR;GITR;TNFRSF18;CD357
目标蛋白:    GITR
UNIPROT ID:    Q9Y5U5
描述:   Recombinant human GITR protein with C-terminal mouse Fc and 6xHis tag
背景:     This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25( )CD4( ) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 50-52 kDa after removal of the signal peptide.
分子特征:   GITR(Gln26-Pro162) mFc(Pro99-Lys330) 6×His
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human GITR Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. HEK293 cell line transfected with irrelevant protein  (B) and human GITR Ligand  (A) were surface stained with Human GITR, mFc-His tagged protein (11147) 1µg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
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