| 货号: 11150 |
| 产品全名: 人 OX40 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 TNFRSF4;OX40;CD134;OX40L receptor;ACT35;TXGP1L |
| 目标蛋白: OX40 |
| UNIPROT ID: P43489 |
| 描述: Recombinant human OX40 protein with C-terminal human Fc and 6xHis tag |
| 背景: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4 T cell response, as well as in T cell-dependent B cell proliferation and differentiation. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 47.2 kDa after removal of the signal peptide. |
| 分子特征: OX40(Leu29-Ala216) hFc(Glu99-Ala330) 6×His tag |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human OX40 Protein, hFc-His Tag on SDS-PAGE under reducing condition. | 
Figure 2. HEK293 cell line transfected with irrelevant protein (B) and human OX40 Ligand (A) were surface stained with Human OX40, hFc-His tagged protein (11150) 1 µg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody. | 
Figure 3. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human OX40 Protein, hFc-His Tag (11150) can bind Anti-OX40 Rabbit mAb in a linear range of 3.2-80 ng/mL. | |
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