| 货号: 11004 |
| 产品全名: 人 CLDN18.2 Full Length 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 Claudin 18.2 |
| 目标蛋白: CLDN18.2 |
| UNIPROT ID: P56856 |
| 描述: 人 CLDN18.2 full length protein-synthetic nanodisc |
| 背景: The protein encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010] |
| 物种/宿主: HEK293 |
| 分子量: The human full length CLDN18.2 Protein has a MW of 27.5 kDa |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure1. Elisa plates were added with Flag Tag CLDN18.2-Nanodisc (0.2 µg/per well) on an anti-Flag monoclonal antibody pre-coated (0.2 µg/per well) plate. Serial diluted anti- CLDN18.2 monoclonal antibody (28078) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-Nanodisc is 593.6ng/ml. | 
Figure2. Human CLDN18.2-Nanodisc, Flag Tag on SDS-PAGE | 
Figure3. Human CLDN18.2-Nanodisc can bind Anti-CLDN18.2 antibody (28078) with an affinity constant of 1.619 nM as determined in a SPR assay. | |
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