武汉费斯德生物科技有限公司， NewEast Biosciences 中国办事处
关于 NewEast Biosciences
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导，包括跨膜受体，例如介导味觉、嗅觉和视觉的那些，（2）核糖体的蛋白质生物合成，（3）调节细胞分化、增殖、分裂和运动，（4）蛋白质通过膜的易位，（5）细胞内囊泡的运输，以及囊泡介导的分泌和摄取，通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力（无需乙酰化）ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。
前 50 名期刊的引用
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- 1. Nature
- 7. Nature Communications
- 8. Cell
- 13. Proceedings of the National Academy of Sciences
- 18. Nucleic Acids Research
- 20. Nature Medicine
- 25. ACS Nano
- 29. Scientific Reports
- 33. PLoS ONE
- 34. Science Advances
- 37. Nature Genetics
- 41. International Journal of Molecular Sciences
- 49. Frontiers in Immunology
NewEast Biosciences Prestained Protein Marker is as accurate as the current non-prestained ladders on the market (Compare lanes 1 and 2 with ours lane 6), but has superior MW precision in comparison with the major market suppliers, ie. Biorad and Thermo (Compare lanes 3, 4 and 5 with ours lane 6). Biorad ladder has two inaccurate bands of 37K and 75K while Thermo prestained products have inconsistent 35K and 25K. Even more, Thermo has many inconsistencies in the molecular weight between its prestained and non-prestained protein markers.
The images are produced from a single controlled experiment. All bands except orange ones are well compatible with near-infrared (NIR) fluorescent system. Note that the red band in Thermo’s product cannot be scanned by NIR fluorescence either. The 货号: of protein markers (left to right) is 22201, 22301, 22202, 22302, 22101, 22203 and 22204.
The membrane (PVDF or NC) after transferring during western blot experiment needs to undergo blocking, primary and secondary antibody incubation, and a multi-step washing process in between. It can take as short as 3 hours or even overnight. In a controlled experiment, our marker and ones of Thermo and Biorad are subjected to 3 hours to overnight washing with TBST washing buffer. After 3 hours, all bands of our products are very clear while the brightness of Thermo’s 10 kD band has been extremely weak and Biorad’s 25K band has completely disappeared, and its 10 KD band is also extremely weak. After overnight washing, only 25K and 20K of our marker are weakened, and the rest of the bands are still very clear. In comparison, Thermo’s 10 KD band is almost completely disappeared, and the two red bands is very faint. More than half of the bands for Biorad product disappears or becomes very faint.
The migration of the prestained marker proteins during electrophoresis depends not only on the proteins themselves but also on the coupled dyes. Therefore, the markers usually show different migration patterns in different electrophoresis systems. The MWs of them are originally calibrated in Tris gel/Tris-Glycine buffer system (Table A). The shifts of the markers in other electrophoresis systems are shown in Table B-D for references. We highly recommend to specifically calibrate their MWs by unstained protein MW standard in corresponding systems other than Tris gel/Tris-Glycine buffer.