武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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BRAF(V600D)

Anti-BRaf(V600D) Mouse Monoclonal Antibody
5/5

¥4,800.00

货号:26184

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   BRAF(V600D) 小鼠单抗
货号:   26184
基因符号:   BRAF, BRAF1, RAFB1
描述:   BRaf(V600D) 小鼠单抗
背景:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
免疫原:   A synthetic peptide from the internal region of BRaf(V600D), human origin.
经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释比:   ELISA 1:1000-1:5000WB 1:500-1:2000IF 1:100-1:200IHC 1:100-1:200
Concentration:    0.5 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes B-Raf V600D, but not wild type B-Raf proteins from vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Immunofluorescence of cells expressing B-Raf proteins with Anti-BRaf(V600D) antibody. HEK293T cells were transfected with pCDNA3-GFP-B-Raf (WT) plasmid (left column) or pCDNA3-GFP-BRaf(V600D) plasmid (right column), then fixed and stained with Anti-BRaf(V600D) monoclonal antibody (货号:26184).

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