| 产品名称: IDH2(R172K) 小鼠单抗 |
| 货号: 26163 |
| 基因符号: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
| 描述: IDH2(R172K) 小鼠单抗 |
| 背景: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas. |
| 免疫原 : A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin. |
| 经过测试的应用: ELISA, WB, IHC |
推荐稀释比:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100
IHC: 1:10-1:100 |
| Concentration: 1 mg/ml |
| 种属反应性: Mouse |
| 形式: Liquid |
| 克隆性： Monoclonal |
| 亚型: |
| 纯化: Purified from ascites |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: recognizes R172K mutant, but not wild type IDH2 of vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:
Western blot analysis of recombinant IDH2(R172K) and wild type proteins.
Purified His-tagged IDH2(R172K) (lane 2) and wild type protein (lane 1) were blotted with Anti-IDH2(R172K) monoclonal antibody (货号:26163).
Immunofluorescence:
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172K) antibody.
HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid or pCDNA3-GFP- IDH2 (R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (货号:26163).
