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IDH2(R172W)

Anti-IDH2(R172W) Mouse Monoclonal Antibody
5/5

¥4,800.00

货号：26164

规格： 100 微升

库存状态：In Stock

           产品描述          
产品名称:   IDH2(R172W) 小鼠单抗
货号:   26164
基因符号:   IDH2(R172W)
描述:   IDH2(R172W) 小鼠单抗
背景:   Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172W IDH2 mutations do exist in diffusely infiltrative gliomas.
免疫原 :   A synthetic peptide from the internal region of IDH2 which includes the mutation of R172W, human origin.
经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释比:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性：  Monoclonal
亚型:   
纯化:   Purified from ascites
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   recognizes IDH1(R172W) of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing

蛋白质印迹:   

Western blot analysis of recombinant IDH2(R172W) and wild type proteins.

Purified His-tagged IDH2(R172W) (lane 2) and wild type protein (lane 1) were blotted with anti IDH2(R172W) monoclonal antibody (货号:26164).

Immunofluorescence:   

Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R172W) antibody.

HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172W) plasmid (right column), then fixed and stained with anti-IDH2(R172W) monoclonal antibody (货号:26164).

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           引用文献          
01. The Diagnostic Utility of IDH2 R172 Immunohistochemistry in Tall Cell Carcinoma With Reversed Polarity of the Breast
Appl Immunohistochem Mol Morphol. 2022  PMID: 36222504
02. Angioimmunoblastic T-Cell Lymphoma and Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma: A Novel Form of Composite Lymphoma Potentially Mimicking Richter Syndrome
Am J Surg Pathol. 2021  PMID: 33739791
03. IDH2 R172 Mutations Across Poorly Differentiated Sinonasal Tract Malignancies : Forty Molecularly Homogenous and Histologically Variable Cases With Favorable Outcome
Am J Surg Pathol. 2021  PMID: 34265800
04. Molecular Pathology of Breast Tumors: Diagnostic and Actionable Genetic Alterations
Surg Pathol Clin. 2021  PMID: 34373096
05. IDH2 Mutation Analysis in Undifferentiated and Poorly Differentiated Sinonasal Carcinomas for Diagnosis and Clinical Management
Am J Surg Pathol. 2020  PMID: 31876581
06. Immunohistochemical analysis of IDH2 R172 hotspot mutations in breast papillary neoplasms: applications in the diagnosis of tall cell carcinoma with reverse polarity
Modern Pathology. 2020  PMID: 31896809
07. Molecular epidemiology of IDH2 hotspot mutations in cancer and immunohistochemical detection of R172K, R172G, and R172M variants
Hum Pathol. 2020  PMID: 33017591
08. DNA methylation-based classification of sinonasal undifferentiated carcinoma
Modern Pathology, 2019  PMID: 31186531
09. The pathological features of angioimmunoblastic T-cell lymphomas with IDH2 R172 mutations
Mod Pathol. 2019  PMID: 30952970
10. The role of a monoclonal antibody 11C8B1 as a diagnostic marker of IDH2-mutated sinonasal undifferentiated carcinoma
Mod Pathol. 2019  PMID: 30206411
11. Vulnerabilities in mIDH2 AML confer sensitivity to APL-like targeted combination therapy
Cell Res. 2019  PMID: 31024166
12. Multiple Ways to Detect IDH2 Mutations in Angioimmunoblastic T-Cell Lymphoma from Immunohistochemistry to Next-Generation Sequencing
J Mol Diagn. 2018  PMID: 29981867
13. Expression of TFH Markers and Detection of RHOA p.G17V and IDH2 p.R172K/S Mutations in Cutaneous Localizations of Angioimmunoblastic T-Cell Lymphomas
Am J Surg Pathol. 2017  PMID: 28945625
14. On the Utility of Short Echo Time (TE) Single Voxel 1H-MRS in Non-Invasive Detection of 2-Hydroxyglutarate (2HG); Challenges and Potential Improvement Illustrated with Animal Models Using MRUI and LCMode
PLoS One. 2016  PMID: 26820720
15. The IDH2 R172K mutation associated with angioimmunoblastic T-cell lymphoma produces 2HG in T cells and impacts lymphoid development
Proc Natl Acad Sci USA. 2016  PMID: 27956631
16. Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
J Neurochem. 2015  PMID: 25251602
17. Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
Brain Tumor Pathol. 2015  PMID: 25324168
18. Mutant IDH inhibits HNF-4a to block hepatocyte differentiation and promote biliary cancer
Nature. 2014  PMID: 25043045
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