Western blot: 

Western blot analysis of recombinant PTEN protein.

Purified His-tagged Survivin protein was blotted with anti survivin mouse monoclonal antibody (货号26131).

Immunohistochemistry:   

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti survivin monoclonal antibody (货号26131). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat mouse IgG (1:50 dilution) was used as secondary antibody.