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GRB7 mAb

anti-GRB7 Mouse Monoclonal Antibody
5/5

$239.00

Cat.#:  26133

   Size:   100 μl

In Stock

          Product Description          

GRB7 Monoclonal Antibody

货号:  26133
基因符号:  GRB7
描述:   GRB7 小鼠单克隆抗体
背景:   Grb7 is an adaptor protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. It plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Grb7 gene is commonly co-amplified (present in excess copies) in breast cancers. Grb7 is over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers. There are clues that it involved in cell migration and metastatic progression of human esophageal carcinomas.
免疫原:   Purified GRB7 protein, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释度:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200
浓度:    0.5 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
Preservative: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes GRB7 of vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing
Western blot:  

Western blot analysis of recombinant GRB7 protein. Purified His-tagged GRB7 protein was blotted with anti GRB7 mouse monoclonal antibody (货号26133).
Immunohistochemistry:  

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti GRB7 monoclonal antibody (货号26133). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat mouse IgG (1:50 dilution) was used as secondary antibody.
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