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FBXW7(R479L)

Anti-FBXW7(R479L) Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26347

   Size:   100 μl

In Stock

          Product Description          
FBXW7(R479L)
货号:  26347
基因符号:  FBW6; FBW7; FBXW7; FBX30; CDC4; SEL10
描述:   FBXW7(R479L) 小鼠单克隆抗体
背景:   F-box/WD repeat-containing protein 7 is a protein that in humans is encoded by the FBXW7 gene. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Mutations in this gene are detected in ovarian, breast and colorectal cancer cell lines, implicating the gene’s potential role in the pathogenesis of human cancers.
免疫原:   A synthetic peptide from the internal region of FBXW7 which includes the mutation of R479L, human origin.
经过测试的应用:   ELISA, WB
推荐稀释度:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000
浓度:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
Preservative: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes R479L mutant, but not wild type FBXW7 of vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant FBXW7(R479L) and wild type proteins. Purified His-tagged FBXW7(R479L) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with FBXW7(R479L) monoclonal antibody (货号26347). Immunofluorescence:   Immunofluorescence of cells expressing FBXW7 proteins with FBXW7(R479L) antibody. HEK293T cells were transfected with pCDNA3-GFP-FBXW7 (WT) plasmid (left column) or pCDNA3-GFP-FBXW7(R479L) plasmid (right column), then fixed and stained with FBXW7(R479L) monoclonal antibody (货号26347).
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