| 货号: S218226 |
| 产品全名: ZNF281 兔多抗 |
| 基因符号 ZBP-99; ZNP-99 |
| UNIPROT ID: Q9Y2X9 (Gene Accession – BC060820 ) |
| 背景: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF281, also known as GC-box-binding zinc finger protein 1, ZBP-99 or ZNP-99 (zinc finger DNA-binding protein 99), is a zinc finger protein that belongs to the Krppel C2H2-type zinc finger protein family. It is expressed ubiquitously at low levels with predominant expression in kidney, liver, lymphocytes and placenta. ZNF281 localizes to the nucleus and contains four C2H2-type zinc fingers. ZNF281 plays a role in repressing the transcription of a variety of genes including Gastrin and ODC (ornithine decarboxylase). In particular, ZNF281 functions by binding to the G-rich box in the enhancer region of the gene. Upon DNA damage, ZNF281 may become phosphorylated by Atm or ATR. |
| 抗原: Fusion protein of human ZNF281 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Stem Cells, Developmental Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 218226(ZNF281 Antibody) at a dilution of 1/30(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 218226(Anti-ZNF281 Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 218226(Anti-ZNF281 Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with fusion protein and then with D223984(Anti-ZNF281 Antibody) at dilution 1/30. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: A549 cell;
Primary antibody: 218226(ZNF281 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds | |
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