| 货号: S218231 |
| 产品全名: ZKSCAN3 兔多抗 |
| 基因符号 ZF47; Zfp47; ZFP306; ZNF306; ZNF309; zfp-47; ZSCAN13; ZSCAN35; dJ874C20.1; dJ874C20.1 |
| UNIPROT ID: Q9BRR0 (Gene Accession – BC006118 ) |
| 背景: Transcriptional factor that binds to the consensus sequence 5′-[GT][AG][AGT]GGGG-3′ and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migration in various tumors and myelomas. |
| 抗原: Fusion protein of human ZKSCAN3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 218231(ZKSCAN3 Antibody) at a dilution of 1/90(Cytoplasm, Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 218231(Anti-ZKSCAN3 Antibody) at dilution 1/90. | 
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 218231(Anti-ZKSCAN3 Antibody) at a dilution of 1/90. | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D223990(Anti-ZKSCAN3 Antibody) at dilution 1/90. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cell, Mouse spleen tissue lysates;
Primary antibody: 218231(ZKSCAN3 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute | |
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