| 货号: S218310 |
| 产品全名: UBE2V1 兔多抗 |
| 基因符号 CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1 |
| UNIPROT ID: Q13404 (Gene Accession – BC000468 ) |
| 背景: Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Alternatively spliced transcript variants encoding multiple isoforms have been described for this gene, and multiple pseudogenes of this gene have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. |
| 抗原: Full length fusion 蛋白 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218310(UBE2V1 Antibody) at a dilution of 1/30(Nucleus ). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218310(Anti-UBE2V1 Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 218310(Anti-UBE2V1 Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D224135(Anti-UBE2V1 Antibody) at dilution 1/30. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human fetal brain tissue, HT-29 cell and Jurkat cell lysates;
Primary antibody: 218310(UBE2V1 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds | |
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