武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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TOP1 Rabbit pAb

Anti-TOP1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S222309

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S222309
产品全名:   TOP1 兔多抗
基因符号   TOPI
UNIPROT ID:   P11387 (Gene Accession – NP_003277 )
背景:   This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
抗原:   Synthetic peptide of human TOP1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 222309(TOP1 Antibody) at a dilution of 1/35(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 222309(Anti-TOP1 Antibody) at dilution 1/35.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 231 and K562 cell lysates;
Primary antibody: 222309(TOP1 Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
           引用文献