| 货号: S220426 |
| 产品全名: STK3/STK4 兔多抗 |
| 基因符号 KRS1; MST2/KRS2; MST1; YSK3; TIIAC |
| UNIPROT ID: Q13043 (Gene Accession – NP_006273 ) |
| 背景: Sterile-20 (Ste20) is a serine/threonine kinase in Saccharomyces cerevisiae that is involved in relaying signals from G protein-coupled receptors to cyto-solic MAP kinase cascades. Mammalian protein kinases that display sequence similarity to Ste20 are divided into two groups, the PAK subfamily and the GCK subfamily. The mammalian Ste20-like kinases (MST kinases), also known as Krs proteins, are members of the GCK subfamily. Ksr-1 (MST-2) and Ksr-2 (MST-1) are both direct substrates of caspase-3 that accelerate caspase-3 activation. |
| 抗原: Synthetic peptide of human STK3/STK4 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 500-2000;ELISA: 2000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human Lymphoma tissue using 220426(STK3/STK4 Antibody) at a dilution of 1/70(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human Lymphoma tissue is first treated with the synthetic peptide and then with 220426(Anti-STK3/STK4 Antibody) at dilution 1/70. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 220426(Anti-STK3/STK4 Antibody) at a dilution of 1/70. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D261514(Anti-STK3/STK4 Antibody) at dilution 1/70. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: PC3 cells, hela cells, human bladder carcinoma tissue, A172 cells, hepG2 cells;
Primary antibody: 220426(STK3/STK4 Antibody) at dilution 1/1000;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds | |
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