武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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SPARC Rabbit pAb

Anti-SPARC Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S216799

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S216799
产品全名:   SPARC 兔多抗
基因符号   ON; ONT; OI17; BM-40
UNIPROT ID:   P09486 (Gene Accession – BC004974 )
背景:   This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
抗原:   Fusion protein of human SPARC
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 1000-5000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Cancer, Cardiovascular, Stem Cells
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216799(SPARC Antibody) at a dilution of 1/35(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216799(Anti-SPARC Antibody) at dilution 1/35.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: U251 and A375 cell lysates;
Primary antibody: 216799(SPARC Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
           引用文献