武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

邮箱:2716620741@qq.com

咨询:027 – 63497286 

SHC1 Rabbit pAb

Anti-SHC1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S218733

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S218733
产品全名:   SHC1 兔多抗
基因符号   SHC; SHCA
UNIPROT ID:   P29353 (Gene Accession – BC014158 )
背景:   This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
抗原:   Fusion protein of human SHC1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Neuroscience
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 218733(SHC1 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 218733(Anti-SHC1 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: Hela, HEPG2, NIH/3T3, 293T, Jurkat, RAW264.7 and 231 cell lysates;
Primary antibody: 218733(SHC1 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
           引用文献