| 货号: S219678 |
| 产品全名: BRAF 兔多抗 |
| 基因符号 NS7; BRAF1; RAFB1; B-RAF1 |
| UNIPROT ID: P15056 (Gene Accession – NP_004324 ) |
| 背景: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome ,x has been identified for this gene. |
| 抗原: Synthetic peptide of human BRAF |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 219678(BRAF Antibody) at a dilution of 1/30(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 219678(Anti-BRAF Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 219678(Anti-BRAF Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with synthetic peptide and then with D260132(Anti-BRAF Antibody) at dilution 1/30. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, Raji cells;
Primary antibody: 219678(BRAF Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute | |
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