| 货号: S220096 |
| 产品全名: BCAR1 兔多抗 |
| 基因符号 CAS; CAS1; CASS1; CRKAS; P130Cas |
| UNIPROT ID: P56945 (Gene Accession – NP_001164185 ) |
| 背景: The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017] |
| 抗原: Synthetic peptide of human BCAR1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 220096(BCAR1 Antibody) at a dilution of 1/50(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 220096(Anti-BCAR1 Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 220096(Anti-BCAR1 Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D260952(Anti-BCAR1 Antibody) at dilution 1/50. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: RAW264.7, PC3, MCF7 cell lysates;
Primary antibody: 220096(BCAR1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute | |
|
