| 货号: S216292 |
| 产品全名: BAG3 兔多抗 |
| 基因符号 BIS; MFM6; BAG-3; CAIR-1 |
| UNIPROT ID: O95817 (Gene Accession – BC014656 ) |
| 背景: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. |
| 抗原: Fusion protein of human BAG3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 40-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 216292(BAG3 Antibody) at a dilution of 1/35(Cytoplasm or Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 216292(Anti-BAG3 Antibody) at dilution 1/35. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 216292(Anti-BAG3 Antibody) at a dilution of 1/35. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D220147(Anti-BAG3 Antibody) at dilution 1/35. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse heart tissue lysate;
Primary antibody: 216292(BAG3 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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