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ARFGAP1 Rabbit pAb

Anti-ARFGAP1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217031

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217031
产品全名:   ARFGAP1 兔多抗
基因符号   ARF1GAP; HRIHFB2281
UNIPROT ID:   Q8N6T3 (Gene Accession – BC028233 )
背景:   The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.
抗原:   Fusion protein of human ARFGAP1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 1000-5000;ELISA: 2000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 217031(ARFGAP1 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 217031(Anti-ARFGAP1 Antibody) at dilution 1/30.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: 293T cells, A549 cells, PC3 cells, NIH/3T3 cells;
Primary antibody: 217031(ARFGAP1 Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
           引用文献