| 货号: S219945 |
| 产品全名: AKAP8 兔多抗 |
| 基因符号 AKAP-8, AKAP95, AKAP 95, AKAP-95 |
| UNIPROT ID: O43823 (Gene Accession – NP_005849 ) |
| 背景: This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. |
| 抗原: Synthetic peptide of human AKAP8 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 219945(AKAP8 Antibody) at a dilution of 1/15(cytoplasm, Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 219945(Anti-AKAP8 Antibody) at dilution 1/15. | 
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 219945(Anti-AKAP8 Antibody) at a dilution of 1/15. | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D260664(Anti-AKAP8 Antibody) at dilution 1/15. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, 293T cells;
Primary antibody: 219945(AKAP8 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes | |
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