| 货号: S222370 |
| 产品全名: HEXB 兔多抗 |
| 基因符号 ENC-1AS; HEL-248; HEL-S-111 |
| UNIPROT ID: P07686 (Gene Accession – NP_000512 ) |
| 背景: Hexosaminidase B is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| 抗原: Synthetic peptide of human HEXB |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 222370(HEXB Antibody) at a dilution of 1/50(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 222370(Anti-HEXB Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 222370(Anti-HEXB Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D264486(Anti-HEXB Antibody) at dilution 1/50. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: A172 cell lysate;
Primary antibody: 222370(HEXB Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 seconds | |
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