| 货号: S220400 |
| 产品全名: BCAS4 兔多抗 |
| 基因符号 CNOL |
| UNIPROT ID: Q8TDM0 (Gene Accession – NP_060313 ) |
| 背景: The gene encoding BCAS4 (Breast carcinoma-amplified sequence 4), a 211 amino acid protein, is found in a region on chromosome 20 that is frequently amplified in human breast cancer. This 20q13 locus amplification is found in 12%?39% of primary breast tumors, which correlates with a 17q23 locus amplification that is found in 20% of primary breast tumors. The amplification and translocation between the BCAS4 gene and the BCAS3 gene, with a 17q23 locus, results in a fusion transcript that is overexpressed in MCF-7 cells. Also, deletion of chromosomal region 20q13.13-q13.2 and resultant deletion of BCAS4, as well as three other genes, is the cause of Okihiro syndrome, a disease characterized by ocular and upper limb anomalies. BCAS4 is normally expressed in thymus, kidney, spleen, placenta and brain. There are three isoforms of BCAS4 which are produced as a result of alternative splicing events. |
| 抗原: Synthetic peptide of human BCAS4 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human gastic cancer tissue using 220400(BCAS4 Antibody) at a dilution of 1/50(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastic cancer tissue is first treated with the synthetic peptide and then with 220400(Anti-BCAS4 Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 220400(Anti-BCAS4 Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D261480(Anti-BCAS4 Antibody) at dilution 1/50. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: K562 and Hela cell lysates, Human left kidney tissue and fetal intestines tissue lysates;
Primary antibody: 220400(BCAS4 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds | |
|
