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NCLN Rabbit pAb

Anti-NCLN Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S218753

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S218753
产品全名:   NCLN 兔多抗
基因符号   NET59
UNIPROT ID:   Q969V3 (Gene Accession – BC013283 )
背景:   May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.
抗原:   Fusion protein of human NCLN
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 30-150;WB: 200-1000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Developmental Biology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 218753(NCLN Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 218753(Anti-NCLN Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and A172 cell lysates;
Primary antibody: 218753(NCLN Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
           引用文献