| 货号: S217993 |
| 产品全名: USP5 兔多抗 |
| 基因符号 ISOT |
| UNIPROT ID: P45974 (Gene Accession – BC004889 ) |
| 背景: Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]). |
| 抗原: Fusion protein of human USP5 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 217993(USP5 Antibody) at a dilution of 1/80(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 217993(Anti-USP5 Antibody) at dilution 1/80. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217993(Anti-USP5 Antibody) at a dilution of 1/80. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D223514(Anti-USP5 Antibody) at dilution 1/80. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: LNCAP cell lysate;
Primary antibody: 217993(USP5 Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds | |
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