| 货号: S217638 |
| 产品全名: NDUFS1 兔多抗 |
| 基因符号 CI-75k; CI-75Kd; PRO1304 |
| UNIPROT ID: P28331 (Gene Accession – BC012068 ) |
| 背景: The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. |
| 抗原: Fusion protein of human NDUFS1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer, Metabolism, Neuroscience |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 217638(NDUFS1 Antibody) at a dilution of 1/35(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 217638(Anti-NDUFS1 Antibody) at dilution 1/35. | 
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 217638(Anti-NDUFS1 Antibody) at a dilution of 1/35. | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D222747(Anti-NDUFS1 Antibody) at dilution 1/35. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: 293T cells, PC3 cells, hela cells, Jurkat cells, Raji cells;
Primary antibody: 217638(NDUFS1 Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds | |
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