| 货号: S217329 |
| 产品全名: HSPA4 兔多抗 |
| 基因符号 RY; APG-2; HSPH2; hsp70; hsp70RY; HS24/P52 |
| UNIPROT ID: P34932 (Gene Accession – BC002526 ) |
| 背景: The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, which include the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The HSP 110 family (also known as the HSP 105 family) is composed of HSP 105, Apg-1 and Apg-2. |
| 抗原: Fusion protein of human HSPA4 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Metabolism, Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 217329(HSPA4 Antibody) at a dilution of 1/25(Cytoplasm or Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 217329(Anti-HSPA4 Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217329(Anti-HSPA4 Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D222187(Anti-HSPA4 Antibody) at dilution 1/25. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Hela cells, Raji cells, NIH/3T3 cells, HepG2 cells, A375 cells;
Primary antibody: 217329(HSPA4 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds | |
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