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ACLY Rabbit pAb

Anti-ACLY Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217192

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217192
产品全名:   ACLY 兔多抗
基因符号   ACL; ATPCL; CLATP
UNIPROT ID:   P53396 (Gene Accession – BC006195 )
背景:   ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
抗原:   Fusion protein of human ACLY
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217192(ACLY Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217192(Anti-ACLY Antibody) at dilution 1/70.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Hela cell lysate;
Primary antibody: 217192(ACLY Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
           引用文献